Hormone levels testing in our clinic supposes that you test the levels of testosterone or human growth hormone in your blood.

Let’s review each type of test in detail.

Testosterone Test

A testosterone test measures the blood level of the male sex hormone testosterone. Testosterone, which plays an important role in sexual development, is produced mainly by the testes in men and in much smaller amounts by the ovaries in women. Testosterone is also produced by the adrenal glands in both women and men.

In young boys, testosterone levels are normally low. As puberty approaches — usually between the ages of 10 and 14 — the pituitary gland (a pea-sized gland near the base of the brain) secretes two hormones (luteinizing hormone, or LH; and follicle-stimulating hormone, or FSH) that work together to stimulate the testes to make testosterone.

Increased testosterone production is what causes men to develop deeper voices, bigger muscles, and body and facial hair. It also helps the testes produce sperm, and it plays a role in speeding growth in height during puberty. Testosterone is also important for bone health.

Two separate assessments may be performed as part of a testosterone test:

  • total testosterone, which measures the entire amount of testosterone in the body, including both the amount bound to proteins that help transport the hormone through the bloodstream and free testosterone;
  • free testosterone, which measures only the testosterone that’s not attached to proteins.

The doctor may order one or both tests. However, because sexual development involves many other hormones, a more complete picture can often be obtained by performing other tests at the same time, including an LH or FSH test.

For example, low levels of testosterone can be due to a problem with the testes’ production of testosterone or to the pituitary gland not making enough of the hormones that stimulate the testes to produce testosterone.

Why It’s Done

Doctors may order a testosterone blood test if a boy appears to be entering puberty earlier or later than expected. High levels are associated with precocious (early) puberty, while low levels may indicate a delay in sexual development. In girls, high levels can be associated with the appearance of masculine characteristics, such as facial hair.

The test may also be used in either men or women to check for damage or disease of the testes, ovaries, adrenal glands, or pituitary gland, or to check for steroid use.

In teens and adults, testosterone levels can help doctors evaluate fertility or menstrual problems and sexual function.


No special preparations are needed for this test. The doctor may want to perform the test in the morning when testosterone levels usually are highest.

On the day of the test, it would be helpful to wear a T-shirt or short-sleeved shirt to allow easier access for the technician who will be drawing the blood.

The Procedure

A health professional will clean the skin surface with antiseptic, and place an elastic band (tourniquet) around the upper arm to apply pressure and cause the veins to swell with blood. Then a needle is inserted into a vein (usually in the arm inside of the elbow or on the back of the hand) and blood is withdrawn and collected in a vial or syringe.

After the procedure, the elastic band is removed. Once the blood has been collected, the needle is removed and the area is covered with cotton or a bandage to stop the bleeding. Collecting blood for this test will only take a few minutes.

What to Expect

Collecting a sample of blood is only temporarily uncomfortable and can feel like a quick pinprick. Afterward, there may be some mild bruising, which should go away in a few days.

Getting the Results

The blood sample will be processed by a machine and the results are commonly available after a few days.


The testosterone test is considered a safe procedure. However, as with many medical tests, some problems can occur with having blood drawn. These include:

  • fainting or feeling lightheaded;
  • hematoma (blood accumulating under the skin causing a lump or bruise);
  • pain associated with multiple punctures to locate a vein.

HGH Testing

HGH test measures the amount of growth hormone (GH) in your blood.

GH is made in your pituitary gland. It affects height, bone, and muscle growth in children. It affects how adults feel and look, as well as their bone and muscle health.

GH is made in a pulse-like manner. Most GH is made while you sleep. When you’re awake, little or possibly no GH is found in your blood. That makes it hard to test your GH level.

Specialists have developed methods to figure out if you make too much or too little by testing your blood over time after you have been given a suppressing agent, such as glucose, or a stimulating agent, like insulin.

Why do I need this test?

You may need this test if your healthcare provider suspects you have a GH problem. Signs include:

  • decreased bone density;
  • reduced muscle strength;
  • increased lipid levels, or fats in your blood.

Acromegaly, or excessive GH disease. Acromegaly refers to excessive enlargement of the limbs from the thickening of bones and soft tissue. This is caused by too much GH. In adults who have stopped growing, the areas most affected are the face, jaw, hands, and feet.

You may also have this test if you have been given GH treatment, so your healthcare provider can watch your dosage and change it if needed.

You may have this test if you are a young adult who has been taking GH for years but may no longer need it. You may also need this test if you have acromegaly, so your healthcare provider can keep an eye on your disease.

What other tests might I have along with this test?

Your healthcare provider may also order a blood test for insulin-like growth factor-1 (IGF-1). GH tells your liver to make IGF-1, so usually, GH and IGF-1 are correlated. GH is secreted in pulses while you sleep, but IGF-1 is always found in your blood. That makes it much easier to find IGF-1 in your blood than GH.

Your healthcare provider may also order other tests to look for a possible growth hormone deficiency. These tests include:

  • Insulin tolerance;
  • growth-hormone-releasing hormone;
  • Insulin-like growth factor-binding protein 3 (IGFBP-3);
  • tests that use other medicines like clonidine or glucagon.

If your healthcare provider suspects that you have excess growth hormone, he or she may order an oral glucose tolerance test or tests of other hormones.

What do my test results mean?

Test results may vary depending on your age, gender, health history, the method used for the test, and other things. Your test results may not mean you have a problem. Ask a healthcare provider what your test results mean for you.

If your levels are higher, it may mean you have:

  • acromegaly in adults;
  • gigantism in children;
  • noncancerous pituitary gland tumor in adults.

If your levels are lower, it may mean you have:

  • growth hormone deficiency;
  • hypopituitarism;
  • noncancerous pituitary gland tumor in adults.

Levels that are higher or lower may also be caused by chronic malnutrition, cirrhosis, and stress from surgery or a serious infection.

How is this test done?

The test is done with a blood sample. A needle is used to draw blood from a vein in your arm or hand. Several samples may be taken over a set period of time.

Does this test pose any risks?

Having a blood test with a needle carries some risks. These include bleeding, bruising, and feeling lightheaded. When the needle pricks your arm or hand, you may feel a slight sting or pain. Afterward, the site may be sore.

What might affect my test results?

Certain foods and beverages, especially those high in protein, can affect your results. Certain medicines, including oral contraceptives containing estrogen, can also affect your results. Great physical or emotional stress and sleeping can affect your results.

How do I get ready for this test?

A healthcare provider may ask you to stop taking oral estrogens for a time before this test. You also may be told to not eat or drink anything but water for a certain amount of time before the test.

Be sure a healthcare provider knows about all medicines, herbs, vitamins, and supplements you are taking. This includes medicines that don’t need a prescription and any illegal drugs you may use.

Fr any additional questions be sure to apply for a free consultation with our qualified expert.